Immunohistopathology of the contralateral testis of rats undergoing experimental torsion of the spermatic cord.
نویسندگان
چکیده
AIM To evaluate the immunohistopathological changes in the contralateral testis of rats after an experimental spermatic cord torsion. METHODS Male Sprague-Dawley rats of 45-50 days old were subjected to a 720 degree unilateral spermatic cord torsion for 10, 30 and 80 days (experimental group, E), respectively or sham operation (control group, C). Histopathology of the contralateral testis as well as germ cell apoptosis were studied using the Terminal Deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL) technique. The number of testicular lymphocytes, mast cells and macrophages, and the expression of tumor necrosis factor-alpha (TNF-alpha) and its receptor (TNFR1) in testicular cells of the contralateral testis were quantified by histochemistry and immunohistochemistry. TNF-alpha concentration in testicular fluid was determined by ELISA. RESULTS In the contralateral testis of rats from the E group, the maximal degree of damage of the germinal epithelium was seen 30 days after torsion. At this time we observed in the E group vs. the C group increases: (i) the number of testicular T-lymphocytes; (ii) the number of testicular mast cells and macrophages; (iii) the percentage of macrophages expressing TNF-alpha; (iv) TNF-a concentration in testicular fluid; (v) the number of apoptotic germ cells; and (vi) the number of TNFR1+ germ cells. CONCLUSION Experimental spermatic cord torsion induces, in the contralateral testis, a focal damage of seminiferous tubules characterized by apoptosis and sloughing of germ cells. Results suggest humoral and cellular immune mediated testicular cell damage in which macrophages and mast cells seem to be involved in the induction of germ cell apoptosis through the TNF-alpha/TNFR1 system and in the modulation of the inflammatory process.
منابع مشابه
Oxidant and Antioxidant Status in Experimental Rat Testis after Testicular Torsion/Detorsion
Background The aim of this study was to determine oxidative stress (OS) parameters after testicular torsion/detorsion in adult rats. MaterialsAndMethods In this experimental study, male adult Wistar rats were divided into four groups, each consisting of seven animals: group I-one hour right testicular torsion with subsequent orchiectomy, group II-one hour right testicular torsion followed by de...
متن کاملDecompressive fasciotomy in testicular torsion of the spermatic cord in rats.
PURPOSE To evaluate the effect of decompression fasciotomy on testes undergoing torsion of the spermatic cord. METHODS Spermatic cord torsion was induced in 40 Wistar testes for six and 12 hours. At the time of distortion, decompression fasciotomy was performed on half of the animals. After 42 days, the testicles were examined both macroscopically and microscopically. A non-parametric Mann- W...
متن کاملThe effect of prophylactic melatonin administration on reperfusion damage in experimental testis ischemia-reperfusion.
AIM Torsion of testis, which is a urologic emergency case, is generally treated by surgical detorsion procedure. However, the resulting reperfusion and both ipsilateral and contralateral testis damage caused thereby are important problems. This study aims at investigating the administration of prophylactic melatonin in order to reduce free radical damage that is caused due to reperfusion after ...
متن کاملSPERMATIC CORD HEMATOMA MIMICKING SPERMATIC CORD TORSION IN A NEONATE
Acute scrotal swelling is an urgent urologic condition, and its failure in diagnosis is "one of the litigious issues in urologic practice. "1-2 Although acute scrotal pain is due to spermatic cord torsion in 25-35% of cases, the urologist should either rule it out or treat it immediately. 2 We operated a case of acute scrotal edema in a two-day-old boy who had, according to clinical results...
متن کامل[Spermatic cord torsion].
Spermatic cord torsion happens rather frequently in young men. It must be discovered early on, to allow for the possibility of removal by surgery. It appears from recently written medical articles that the endocrine function of the testis is protected following several hours of torsion and the risk of immunological damage to the contralateral testis is subsequently reduced.
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ورودعنوان ژورنال:
- Asian journal of andrology
دوره 8 5 شماره
صفحات -
تاریخ انتشار 2006